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M94A3256.TXT
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1994-10-25
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Document 3256
DOCN M94A3256
TI Amplification of viral replication relevantly with reverse
differentiation in HIV-1 infected cells with macrophage phenotype.
DT 9412
AU Okada Y; Kameoka M; Kimura T; Kishi M; Ikuta K; Institute of
Immunological Science, Hokkaido University, Sapporo,; Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):118 (abstract no. PA0091). Unique
Identifier : AIDSLINE ICA10/94369319
AB OBJECTIVE: The cells of monocyte-macrophage lineage was important as
HIV-1 reservoir, especially for understanding the persistent mechanism
in asymptomatic carriers. Here, we examined the HIV-1 life cycle after
the infection of differentially differentiated cells. METHODS: U937
cells were differentially differentiated by varying dose of PMA for 3
days, then infected with HIV-1. Thereafter, the cells were sequentially
examined for cell viability, morphology, expression of differentiation
antigens, superoxide generation, and HIV-1 phenotypes. RESULTS: The
PMA-treated cells were dose-dependently differentiated to adhered
morphology. HIV-1 replication after infection in these cells reversely
correlated with PMA dosages, i.e. the replication rate was increasingly
higher with lower dose of PMA. Thereafter, reverse differentiation
(retro-differentiation) of these cells from adhered to floating
morphology led to amplification of viral replication. Cells that were
well differentiated with higher dose of PMA were hard to indue the
retro-differentiation. DISCUSSION AND CONCLUSION: These results indicate
a close correlation of HIV-1 amplification and cell differentiation
level in the monocyte-macrophage lineage. The retro-differentiation
phenomenon in the infected cells seems to be particularly important for
understanding viral activation mechanism in reservoir cells. Intrinsic
redox regulation or cytokines induced by opportunistic infections may
involve in the stimulation of the cells.
DE Cell Differentiation/DRUG EFFECTS Cell Line Dose-Response
Relationship, Drug Human HIV Infections/*MICROBIOLOGY/PATHOLOGY
HIV-1/GROWTH & DEVELOPMENT/*PHYSIOLOGY Macrophages/DRUG
EFFECTS/*MICROBIOLOGY/PATHOLOGY Monocytes/DRUG
EFFECTS/MICROBIOLOGY/PATHOLOGY Phenotype Tetradecanoylphorbol
Acetate/ADMINISTRATION & DOSAGE/PHARMACOLOGY *Virus Replication/DRUG
EFFECTS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).